Purification and properties of an .ALPHA.-glucosidase (glucoamylase) in sugar beet seed.

Purification and properties of an amylopullulanase, a glucoamylase, and an alpha-glucosidase in the amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum.

Thermoanaerobic bacteria are of considerable interest as producers of thermostable amylolytic enzymes. The soluble amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum DSM 571 was fractionated into a pullulanase, a glucoamylase, and an alpha-glucosidase. The enzymes were purified to homogeneity and their physical and catalytic properties were studied. The pullulanase, which c...

Purification of an acid alpha-glucosidase by dextran-gel filtration.

1. The behaviour of rat liver alpha-glucosidases on dextran gel (Sephadex G-100) columns was studied. A ;retardation' of an acid alpha-glucosidase activity was observed. This activity was identified as lysosome alpha-(1-->4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid alpha-glucosidase of human ...

Purification and Properties of an Inducible p-Glucosidase of Yeast*

In the past decade considerable advances have been achieved in the study of induced enzyme biosynthesis in microorganisms. A comparison of three of the better studied systems, P-galactosidase in Escherichia coli (l), penicillinase in Bacillus cereus (2), and oc-glucosidase in Xaccharomyces cerevisiae (3) indicates that there are a number of differences in these inductions. In order to compare f...

Purification and properties of an inducible beta-glucosidase of yeast.

In the past decade considerable advances have been achieved in the study of induced enzyme biosynthesis in microorganisms. A comparison of three of the better studied systems, P-galactosidase in Escherichia coli (l), penicillinase in Bacillus cereus (2), and oc-glucosidase in Xaccharomyces cerevisiae (3) indicates that there are a number of differences in these inductions. In order to compare f...

Effect of Drying Conditions on Properties of Dried Sugar Beet